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Neuroblastoma × spinal cord (NSC) hybrid cell lines resemble developing motor neurons
Neil R. Cashman,
Heather D. Durham,
Jan Krzysztof Blusztajn,
Kenichiro Oda,
Takeshi Tabira,
Ivan T. Shaw,
Simone Dahrouge,
Jack P. Antel,
Corresponding Author
Neil R. Cashman
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Montreal Neurological Institute, 3801 University Street, Montreal, Quebec, Canada H3A 2B4Search for more papers by this authorHeather D. Durham
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Search for more papers by this authorJan Krzysztof Blusztajn
Department of Pathology, Boston University School of Medicine, Boston, Massachusetts 02118
Search for more papers by this authorKenichiro Oda
National Institute of Neuroscience NCNP, Kodaira, Tokyo 187, Japan
Search for more papers by this authorTakeshi Tabira
National Institute of Neuroscience NCNP, Kodaira, Tokyo 187, Japan
Search for more papers by this authorIvan T. Shaw
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Search for more papers by this authorSimone Dahrouge
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Search for more papers by this authorJack P. Antel
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Search for more papers by this authorNeil R. Cashman,
Heather D. Durham,
Jan Krzysztof Blusztajn,
Kenichiro Oda,
Takeshi Tabira,
Ivan T. Shaw,
Simone Dahrouge,
Jack P. Antel,
Corresponding Author
Neil R. Cashman
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Montreal Neurological Institute, 3801 University Street, Montreal, Quebec, Canada H3A 2B4Search for more papers by this authorHeather D. Durham
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Search for more papers by this authorJan Krzysztof Blusztajn
Department of Pathology, Boston University School of Medicine, Boston, Massachusetts 02118
Search for more papers by this authorKenichiro Oda
National Institute of Neuroscience NCNP, Kodaira, Tokyo 187, Japan
Search for more papers by this authorTakeshi Tabira
National Institute of Neuroscience NCNP, Kodaira, Tokyo 187, Japan
Search for more papers by this authorIvan T. Shaw
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Search for more papers by this authorSimone Dahrouge
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Search for more papers by this authorJack P. Antel
Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada H3A 2B4
Search for more papers by this authorAbstract
We have developed a series of mouse-mouse neural hybrid cell lines by fusing the aminopterin-sensitive neuroblastoma N18TG2 with motor neuron-enriched embryonic day 12–14 spinal cord cells. Of 30 neuroblastoma-spinal cord (NSC) hybrids displaying a multipolar neuron-like phenotype, 10 express choline acetyltransferase, and 4 induce twitching in cocultured mouse myotubules. NSC-19, NSC-34, and their subclones express additional properties expected of motor neurons, including generation of action potentials, expression of neurofilament triplet proteins, and acetylcholine synthesis, storage, and release. In addition, NSC-34 cells induce acetylcholine receptor clusters on cocultured myotubes, and undergo a vimentin-neurofilament switch with maturation in culture, similar to that occurring in neuronal development. NSC cell lines appear to model selected aspects of motor neuron development in an immortalized clonal system. © 1992 Wiley-Liss, Inc.
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